The evaluation of an organization’s performance may also consider the assessment of inter-organizational relationships (IORs). However, the evaluation of IORs is typically based on success factors, such as trust, which are difficult to be measured quantitatively. In this paper, we present a framework supporting inter-organizational performance evaluation which integrates (i) a bottom-up approach supporting the identification of Key Performance Indicators (KPIs) from business information, event logs, as well as process models, and (ii) a top-down approach for measuring business performance on the strategic level based on the Balanced Scorecard (BSC) method. In order to prove the feasibility of the framework, we present an inter-organizational performance analysis case study of a beverage manufacturing company. The case study shows that the framework enables (i) the derivation of quantifiable KPIs from operational data and (ii) the alignment of KPIs with business objectives allowing an evaluation of IORs on the strategic level.